Crispr/Cas9 U赢电竞毒

高效极速基因敲除神器-Crispr/Cas9 U赢电竞毒



? ? ? Crispr/Cas9 简介

CRISPR (clustered, regularly interspaced, short palindromic repeats)是一种来自细菌降解入侵的病毒 DNA 或其他外源 DNA 的免疫机制。目前,来自Streptococcus pyogenes 的CRISPR-Cas9系统应用最为广泛。Cas9 蛋白(含有两个核酸酶结构域,可以分别切割DNA 两条单链。Cas9首先与crRNA及tracrRNA结合成复合物,然后通过PAM序列结合并侵入DNA,形成RNA-DNA复合结构,进而对目的DNA双链进行切割,使DNA双链断裂。


通过基因工程手段对crRNA和tracrRNA进行改造,将其连接在一起得到sgRNA(single guide RNA)。融合的RNA具有与野生型RNA类似的活力,但因为结构得到了简化更方便研究者使用。通过将表达sgRNA的原件与表达Cas9的原件相连接,得到可以同时表达两者的质粒,将其转染细胞,便能够对目的基因进行操作。














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? ?5Suppression of KRas-mutant cancer through the combined inhibition of KRAS with PLK1 and ROCK
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? ?7LRP6 acts as a scaffold protein in cardiac gap?junction assembly
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? ?12Interleukin-1β induces autophagy by affecting calcium homeostasis and trypsinogen activation in pancreatic acinar cells
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? ?13MicroRNA-1 functions as a potential tumor suppressor in osteosarcoma by targeting Med1 and Med31
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? ?14MicroRNA-216b/Beclin 1 axis regulates autophagy and apoptosis in human Tenon's capsule fibroblasts upon hydroxycamptothecin exposure
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? ?17Ulinastatin Reduces the Resistance of Liver Cancer Cells to Epirubicin by Inhibiting Autophagy
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? ?23Effects of insulin-like growth factor-1 on the assembly and secretion of very low-density lipoproteins in cow hepatocytes in vitro
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? ?35miR-186 inhibits cell proliferation in multiple myeloma by repressing Jagged1
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? ?38Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1
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? ?39A novel role of microRNA 17-5p in the modulation of circadian rhythm
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? ?40Excess iodine promotes apoptosis of thyroid follicular epithelial cells by inducing autophagy suppression and is associated with Hashimoto thyroiditis disease
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